Study Effect of BA, GA3 Hormone and Light Rate of Poinsettia under In-Vitro Condition

ABSTRACT: Slow growth rate of plantlets, few micro-shoots per explant, and slow root growth rate are restrictions of in vitro propagation of poinsettia (Euphorbia pulcherrima Willd. Ex Koltz). In order to study the effects of benzyladenine (BA) and giberlin (GA3) hormone management in vitro culture of poinsettia (Euphorbia pulcherrima wild), an experiment as factorial in RCBD with three replications conducted during 2010 at Research Laboratory of Faculty of Agriculture, Lahijan University in Iran. Factors were BA hormone levels (B1=0.01, B2=0.1, B3=1 and B4=5 mg/L), GA3 hormone levels (G1= 0, G2= 1 and G3= 5 mg/L), and light rate (L1= 16h light- 8h dark and L2= 0h light- 24h dark). The culture beds were contained MS, sucrose (3%) and agar (75%). The results show that, BA hormone levels, GA3 hormone levels and light rate on shoot length in vitro culture of poinsettia had a significant difference in 1 % probability level. The highest shoot length in micro cuttings of poinsettia were obtained with application 0.1 mg/L BA hormone (14.12 mm), 0.1 mg/L BA hormone (15.85 mm) and full dark condition (11.51 mm).