Author/Authors :
J، Hussaini نويسنده Faculty of Medicine, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia , , M. H. M، Nazmul نويسنده Faculty of Medicine, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia , , M. A، Abdullah نويسنده Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia , , S، Ismail نويسنده Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia ,
Abstract :
In this study the potential of the previously contracted recombinant clone ABA392 derived from Pasteurella multocida serotype B to protect laboratory animal against haemorrhagic septicaemia was determined. After reconfirmation of plasmid DNA size, its stability and pathological effect of the clone, passive mice protection test and active immunization was carried out. Pooled serum sample from rats vaccinated with live and killed form of the clone was administered to mice and provided 66% protection while active immunization with the recombinant clone conferred 83% immunity to mice when challenged with lethal dose of P. multocida. ELISA results were positive for presence of antibody in serum of immunized mice. Sub-cloning of the insert ABA392 into an expression vector pQE32 was carried out to express its protein. It was found that the recombinant clone ABA392 is immunogenic and could be used as vaccine in future.
