Hygromycin B - An Alternative in Flax Transformant Selection

In Solanum nigrum, the initiation of root primordia occurred directly on stem explants or microshoots cultured on half strength MS medium supplemented with 1 mg dm-3 indolebutyric acid (IBA), indole-3-acetic acid, or naphthalene acetic acid, IBA being most effective. Initiation of shoot primordia occurred on B5 medium supplemented with 0.5 mg dm-3 benzylaminopurine (BAP) or kinetin; BAP was more active. During shoot formation, the increased content and the expression of new isoenzymes of peroxidase (POX), esterase and malate dehydrogenase was found. On the other side, expression of new indophenol peroxidase isoenzyme was detected during root formation. Organogenesis of S. nigrum has no effect on glutamate oxaloacetate transaminase and glutamate dehydrogenase content. Differential expression of POX could be detected between basal and upper side of explants.