Identification of three species of genus Allium using DNA barcoding

ABSTRACT: DNA barcoding is a relatively new method developed to provide rapid, accurate and automatable species identification using standardized DNA sequences as tags. Species identification in this way is usually achieved by the repossession of a short DNA sequence, the “barcode” from a standard part of the genome of a given species. An experiment was conducted to identify three species of genus Allium using five barcode regions including ndhJ, rpoC1, rpoB, YCF5 and rbcL. Results indicated that A. ampeloprasum persicum was the only identified species. Barcode region of YCF5 had the highest SRSP with 93.33%. The highest interspecific diversity and intraspecific divergence with 0.8% and 0.4% were observed in region of ndhJ which was the most sufficient barcode region for species identification.