Purification and Properties of a Ribonuclease from Cowpea Cotyledons

Leaf tissues of 38 genotypes, derived from four accessions, of the hexaploid species Helianthus tuberosus (2n=6x=102) responded to growth regulators (BA, NAA) chiefly by forming callus, while aventitious organogenesis or somatic embryogenesis were induced occasionally. A remarkable regeneration frequency (about 30 %) was achieved only from leaves of genotype HTPI-15. Explants of many regenerated plants of HTPI-15 subjected to a second culture cycle in vitro displayed a high morphogenetic potential (regeneration frequency > 90 %). White globular structures were initiated on the adaxial surface of these leaves without a callus phase. Somatic embryogenesis was asynchronous and embryoids, of different developmental stage, were simultaneously detected on each explant. Although many embryos developed single or malformed cotyledons or germinated precocciously, without the differentiation of a complete root system, phenotypically normal plants were regenerated after rooting on regulator-free half-strength MS medium.