Association of milk traits with SSCP polymorphisms at the growth hormone gene in the Serrana goat

The present study suggests the existence of an association between milk production traits and genetic polymorphisms at the growth hormone (GH) gene in the Portuguese indigenous Serrana goat. TheDNAfrom 229 animals of two ecotypes (Jarmelista and Ribatejano)was analysed by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique revealing a high degree of genetic polymorphism at the goat GH (gGH) gene. Two conformation patterns were detected in exons 1 and 2, 6 in exon 3, 10 in exon 4 and 5 in exon 5. The evaluation of an association effect between these SSCP patterns with milk, fat and protein yields and fat and protein percentages suggests a positive effect of pattern A/B of exon 4 for Ribatejano ecotype and of pattern A/B of exon 2 for Jarmelista ecotype with milk yield (P < 0.05) and of pattern A/B of exon 1 and pattern B/B of exon 2 with protein percentage (P < 0.05) for Ribatejano ecotype. The results support the hints suggested by previous studies of the importance of the gGH gene as a candidate gene for marker-assisted selection in goat breeds and suggest that exon 4 is a preferential target for further investigation on mutations that influence milk yield variation. © 2003 Elsevier Science B.V. All rights reserved