Abstract :
To assess the possible role of the “brain isoenzyme” of creatine kinase (CK-BB) as an early
diagnostic tools for cerebral coenurosis we examined sera from 68 sheep (10–13 months
old) from a flock on which cerebral coenurosis was endemical. After sampling, sheep were
slaughtered, and neurological lesions were classified as purulent-necrotic migratory lesions
or cystic lesions. The discriminating power of CK-BB to diagnose cerebral coenurosis was
assessed using a receiver operating characteristic (ROC) curve.
The prevalence of cerebral coenurosis was 39.7% (27/68). Migratory lesions were found
in 23 sheep (85.1%), cystic lesions in 3 sheep (11.2%) and both lesions were found in 1 sheep
(3.7%). The most abundant isoenzymes detected both in controls and in sheep with cerebral
coenurosis were CK-MM, Macro-CK2 and CK-BB. No significant differences regarding
absolute values of CK-BB were found suggesting that CK-BB is not released in serum during
cerebral coenurosis. Conversely, CK-MM was significantly lower (P < 0.05) in sheep with
cerebral coenurosis compared with controls, inducing a relative increase of the percentage
of CK-BB (P < 0.05). This latter increase had a good discriminating power (area under
the ROC curve: 0.71; P < 0.000). Although at its best diagnostic performance (CK-BB higher
than 24,4%) sensitivity and specificity were moderate (66.7% and 68.3%, respectively), the
likelihood ratio of increased CK-BB was 2.10. The increased percentage of CK-BB can thus
be useful in the diagnostic approach to suspected cases of cerebral coenurosis, when the
pre-test probability of disease is high.
