Abstract :
The blood levels of cholinesterase reactivator pralidoxime (2-PAM) were determined in
goats following single dose intravenous administration@30 mg/kg body weight injected as
6% freshly prepared solution. Blood and urine samples were collected at different predetermined
time intervals and 2-PAM was analysed by spectrometric method with the minimum
detection of 1.0 gml−1. The peak plasma concentration was 49.52±3.99 gml−1 at 2 min
post administration which rapidly declined to 15.53±2.11 gml−1 at 10 min. Thereafter,
it gradually disappeared to 1.33±0.41 gml−1 at 1.5 h. The pharmacokinetic parameters
were determined by employing two-compartment open model. The t1/2˛, t1/2ˇ, Vdarea and
ClB were calculated to be 1.68±0.35 min, 21.17±1.65 min, 1277.95±195.67 ml/kg and
41.17±3.66 ml/kg/min, respectively. Approximately52%of the total administered dosewas
eliminated in urine within 24 h. The plasma protein binding was estimated by equilibrium
dialysis technique. The in vitro plasma protein binding of 2-PAM was 64.7%.
Based on these data, a satisfactory intravenous dosage regimen of 2-PAM in goats would
be 38 mg/kg body weight repeated at hourly intervals.
