Title of article :
Characterization of caprine embryonic stem cell-like outgrowths
derived from the inner cell mass isolation
Author/Authors :
S.Y. Goh، نويسنده , , R.B. Abdullah?، نويسنده , , W.E. Wan Khadijah، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2012
Abstract :
Embryonic stem (ES) cells have limitless potential in the field of biological sciences and
regenerative medicine due to their pluripotency and ability for indefinite self-renewal. The
aims of this study were: (i) to isolate goat ES cell lines from both in vivo and in vitro derived
blastocysts and (ii) to characterize the growth characteristics and expression of markers
indicative of pluripotency in goat ES cells. The goat inner cell mass (ICM) of in vitro and in vivo
derived whole blastocysts was isolated by manually cutting or laser dissection, with in vitro
cultured mouse embryonic fibroblasts (MEF’s) as a feeder layer to obtain the ES cells. The
in vivo derived blastocysts recorded a significant difference in producing goat ES cell lines
at Passage 3, compared with in vitro derived blastocysts (91.7% vs. 20.8% respectively). The
manually cut ICM (inner cell mass) isolation technique consistently recorded the highest
success rate in goat ES cells for Passages 1 and 3, compared with the whole blastocyst culture
(control) and those from the laser dissection technique (71.3% vs. 39.6% and 43.9%; 35.0%
vs. 12.5% and 23.3% respectively). Alkaline phosphatase (AP), Oct-4 and SSEA-3 staining
gave positive results in identifying the goat ES cells. In conclusion, goat ES cells could be
produced by isolation of the ICM by using both manual and laser dissection of blastocysts,
as characterized by AP, Oct-4 and SSEA-3 staining.
Keywords :
GoatES cellICM isolationIn vitroIn vivo
Journal title :
Small Ruminant Research
Journal title :
Small Ruminant Research