Abstract :
Set-type yoghurts were produced from goat’s milk cross-linked by microbial transglutaminase
(TGase) using different amounts (1, 2 or 3 U/g protein), and the control yoghurt did
not contain any TGase. Fresh and stored yoghurts (14 days) were examined for sensory profiling,
instrumental texture analysis, syneresis and microstructure to study the influence of
TGase concentration and storage time on the quality of the experimental products. TGase
yoghurts (fresh and stored) had higher sensory scores, higher readings for texture measurements
(i.e. firmness) and a lower volume of syneresis than the control product. In general,
the higher the amount of added TGase used in the milk base the firmer the fresh yoghurt
became, the lowest syneresis was observed and the highest sensory score was awarded by
the taste panellist. Nevertheless, the best sensory score of fresh yoghurt was awarded to
the product made with added 2 U TGase/g protein, and for stored yoghurts containing 2 and
3 U TGase/g protein, but syneresis of the product containing 3 U TGase/g protein was higher
than the other experimental yoghurts. The firmness of the fresh experimental yoghurts (2
and 3 U TGase/g protein) was similar, and the stored products exhibited slight tendencies
to syneresis and the firmness of the product was slightly higher, but the sensory score was
lower. The protein matrix of the experimental yoghurts was more compact, and consisted
of smaller casein micelles/aggregates than those of the control yoghurt. Modification of the
goat’s milk with microbial TGase improved the sensory quality, textural characteristic, and
reduced syneresis of set-yoghurt when compared with the control product. Overall, the
results suggest that best set-type goat’s milk yoghurt was made using 2 U TGase/g protein.