A Fokker–Planck framework in the PolyC:PolyG denaturation

In this work the author presents a Fokker–Planck formalism to describe the denaturation process for simple DNA chains as PolyC:PolyG. This model consists in simulating the denaturation as a diffusion process of a virtual particle moving in an effective external potential. Assuming that such potential is harmonic, we obtain the melting temperature for a PolyC:PolyG chain. Under this assumption the melting temperature Tm, becomes a function of two parameters which specify the nature of the effective potential. The values of the parameters which lead to an agreement between the experimental and theoretical melting temperatures for a PolyC:PolyG chain immersed in a solution of 19.5 mM of Na+ and pH 7 are reported