Author/Authors :
Hassani، Fatemeh نويسنده , , Eftekhari-Yazdi، Poopak نويسنده Baghaban Eslaminejad, M.R. , Karimian، Leila نويسنده Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , , Rezazadeh Valojerdi، Mojtaba نويسنده , , Movaghar، Bahar نويسنده Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , , Fazel Zarandi، Mohammad Hossein نويسنده Professor of Department of Industrial Engineering, Tehran, Iran , , Fouladi، Hamid Reza نويسنده Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , , Shabani، Fatemeh نويسنده , , Johansson، Lars R. K. نويسنده ,
Abstract :
Background: The aim of this study is to investigate the effect of ISM1 culture medium
on embryo development, quality and outcomes of in vitro fertilization/intracytoplasmic
sperm injection (IVF/ICSI) cycles. This study compares culture medium commonly used
in the laboratory setting for oocyte recovery and embryo development with a medium
from MediCult. We have assessed the effects of these media on embryo development and
newborn characteristics.
Materials and Methods: In this prospective randomized study, fertilized oocytes
from patients were randomly assigned to culture in ISM1 (MediCult, cycles:
n=293) or routine lab culture medium (G-1
v5; Vitrolife, cycles: n=290) according
to the daily media schedule for oocyte retrieval. IVF or ICSI and embryo
transfer
were performed with either MediCult media or routine lab media. Embryo
quality
on
days
2/3, cleavage,
pregnancy
and
implantation rates,
baby
take home
rate
(BTHR), in addition to the weight and length of newborns were compared
between
groups.
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Results: There were similar cleavage rates for ISM1 (86%) vs. G-1
v5 (88%). We
observed a significantly higher percentage of excellent embryos in ISM1 (42.7%) compared
to
G-1
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v5 (39%, p < 0.05). Babies born after culture in ISM1 had both higher
birth weight (3.03 kg) and length (48.8 cm) compared to G-1
v5 babies that had a birth
weight of 2.66 kg and a length of 46.0 cm (p < 0.001 for both).
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Conclusion: This study suggests that ISM1 is a more effective culture medium in
generating higher quality embryos, which may be reflected in the characteristics of
babies at birth.