Increasingofalpha-tocopherol in cell suspension cultures ElaeagnusangustifoliaL

ABSTRACT: Plants are a tremendous source for the discovery of new medicinal valuable products for drug development. The evolving commercial importance of secondary metabolites has in recent years resulted in a greatinterest in secondary metabolism, particularly in the possibility of altering the production of bioactive plant metabolites by using of tissue culture technology. The presentinvestigation assaytoincrease accumulation of alpha-tocopherol in cell suspension culture of Elaeagnusangustifolia.callusproducted wastransfered to suspension culture medium ,then Jasmonic acid ( with 5, 25,50 µm)and salicylic acid (with 0.1, 0.5, 1 mM concentrations )elicitors added to samples. HPLC instrument was used to evaluated the rate of alpha tocopherolproduction. Our results showed both of elicitors effective in increasingalpha tocopherolproduction.The 1mM concentration of SA was most effective in alpha tocopherol production in the cell cultures. The increased concentration of SA was positively corrected with alpha tocopherol accumulation.