Response to Tissue Culture in Four Canola Cultivars through Hypocotyl Explants

One of the alternative methods for enhancing or modifying a specific property without employing classical techniques is genetic engineering, which reduces the time necessary for a new variety production. Tissue culture is one of the technical prerequisites for genetic engineering. The present research aimed to investigate the effective parameters involved in tissue culture of canola plants and to develop an applicable protocol for mature plant regeneration. This regeneration was experimented via hypocotyl explants by four canola cultivars (Talaye, Okapi, Hyola 401, and RGS003), seven different pretreatment media, three treatments on the duration of keeping in light/dark, and four treatments on different explant ages. The data related to the regeneration of plantlet were gathered and analyzed by SAS software. The results showed that between different experimented treatments, the highest percentages of regeneration (%55) are related to 10-day-old hypocotyl explants for Hyola 401 in a M3 pretreatment medium containing NAA (0.1 mg/l), and to RGS003 (%46.65) in a M6 pretreatment medium containing NAA (0.1 mg/l) +Kin (0.5 mg/l) with the age of 10 days. The explants with the age of fewer than 15 days had the better response to tissue culture with respect to regeneration esp. the stem number above and below 1.5 cm. Moreover, the highest number of the stems below 1.5cm was obtained for Hyola 401 in medium M3 (3.5 n) and for RGS003 in medium M6 (2.5 n). The attained protocol benefits from a high level of repeatability and can be employed for regeneration and transformation of canola plants.