Investigation of MUCIN1 gene polymorphism by PCR-RFLP in buffalo population in khouzestan province

Mucin1 is one of mucin gene family located on the cell membrane. mucin membrane First was found as a molecular detection single colony antibodies of mouse cells surface. Mucin1 has a major role in drugs delivery and another therapeutic system. According to studies mucin1 has a direct effect on important economical traits such as milk and fatty milk production, fertility, Pregnancy, carcass traits and the ressistance aginest disseses. In the reproductive tract of the female animal mucin has an important role in controlling embryo implantation. Mucin protein role in uterine can be a sign of fertility differences. Animals that contain the gene and more sequences of mucin gene are superior than others in reproductive traits and the acquired immune and resistance to diseases. This gene is located on buffalo chromosome 3. In order to study mucin gene polymorphism 80 buffalo in the khozestan province were randomly bled. DNA extracted from blood samples and polymerase chain reaction (PCR) amplified fragment of 300 bp of gene was performed. DdeI Restricted enzymes were used for enzymatic digestion. Based on the results of the frequency of genotype AA was 100 percent and the frequency of AB, BB were zero. A allele frequency in this population is1 and B allele frequency is zero. Because of selection population size, mutation, migration and genetic drift the hardy-weinberg equilibrium in the Buffalo herd was not established