Effect of Detergents and Chaotropic Agents on Kinetic Properties of Polyphenol Oxidase of Solanum Lycopersicum

Browning of damaged tissues in fresh fruits and vegetables results mainly from the oxidation of phenolic compounds to quinones by polyphenol oxidase (PPO). The activation of PPO by sodium dodesyl sulphat investigated, but reports on the effect of detergents and chaotropic agents on activity of PPO are scarce. Here the effect of some detergents and chaotropic agent on activity of enzyme in soluble and in partial purified fraction was investigated. Sarkosyl activated activity of enzyme for oxidation of catechol and pyrogallol, although has inhibitory effect in higher concentrations. Other ionic, Nonionic detergents and Chaotropic agents showed inhibitory effect on activity of PPO in soluble and in partial purified fraction. Inhibitory effect of Urea, GnHcl, NP-40, Triton x-100 and Sodium cholate in presence of catechol is more than these effect in presence of pyrogallol. On the other hand, sodim cholate and NP-40 has more inhibitory effect on PPO activity in presence of pyrogallol at high concentrations. The same effects were obtained on the partially purified enzyme. Results identified and confirmed the differences in structure and conformation of enzyme in soluble and in partial purified fraction for oxidation of different substrates.