Author/Authors :
Aminsharifi، Alireza نويسنده , , Ghaderi، Abbas نويسنده , , Naeimi، Sirous نويسنده Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran , , Khezri، Abdolaziz نويسنده , , Agahi، Neda نويسنده Department of Urology, Shiraz University of Medical Sciences, Shiraz, Iran , , Aminian-Modarres، Amir Masoud نويسنده Department of Electrical Engineering , , Zamiri، Nima نويسنده ,
Abstract :
Introduction: Cytotoxic T-cell lymphocyte antigen 4 (CTLA-4) is a member of
the superfamily of immunoglobulins that are mainly expressed by activated T cells.
It is established that blockade of CTLA-4 receptors leads to the enhancement of an
immune response. Different polymorphisms of the CTLA-4 gene have been described
which cause increased susceptibility to various malignancies such as lymphoma or
multiple myeloma. Considering that bladder cancer is one of the most prevalent cancers
worldwide, we have evaluated the role of CTLA-4 gene polymorphism at position
+49 A/G in the formation or progression of bladder cancer in southern Iran.
Materials and Methods:Atotal of 226 individuals between February 2005 and
June 2006 were included and placed into two subgroups: patients diagnosed with
bladder cancer and a control group. Demographic data and risk factors were collected
from both groups. The DNAof all subjects was extracted from their blood samples.
Different genotypes of the CTLA-4 gene were determined using the restriction
fragment length polymorphism (RFLP) technique and data were compared in both
groups by using Pearsonʹs chi-square test.
Results: The prevalence of AA, AG and GG genotypes at position 49, according
to the PCR-RFLP method, were 57.5%, 37.2% and 5.3% in the control group,
respectively. In the patient group, the prevalence of these genotypes was: AAin 57.5%,
AG in 32.7% and GG in 9.8%. Statistical analysis of data showed no significant
difference in both groups (P value=0.40). Also there was no correlation between
different genotypes of the CTLA-4 gene and invasiveness of the disease in our
cases.
Conclusion: Although polymorphism of the CTLA-4 gene at position 49 of
exon-1 increases susceptibility to several malignancies, our study showed no
relationship between polymorphism at this position and genetic susceptibility to the
development of bladder cancer, nor was there any association with disease progression.
