Author/Authors :
Gavanji، Shahin نويسنده , , Jalali Zand، Alireza نويسنده Department of Plant protection, Khorasgan (Isfahan) Branch , Islamic Azad University, Isfahan , Iran , , Larki، Behrouz نويسنده , , mojiri، Amin نويسنده School of Civil Engineering, Engineering Campus, University Sains Malaysia, 14300 Nibong Tebal, Penang, Malaysia , , Bakhtari، Azizollah نويسنده Isfahan University of Technology, Department of Animal Science, Iran, Animal Science , , kashkouli، Marzieh نويسنده Department of Plant Protection, College of Agriculture, Isfahan University of Technology ,
Abstract :
Many transition metals are essential for the nutrition of plants. However, because of the potential toxicity, strict regulation of these ions is also critical for plant cells. In order to maintain metal homeostasis, plants have evolved diverse mechanisms to ensure appropriate concentrations of essential metal ions, as well as to remove non-essential forms by controlling their accumulation, distribution and sequestration. In this study, at first the Metal transporter Nramp1 was prepared from NCBI website. Then the sequence of the protein had been compared to Metal transporter Nramp1 sequences in different plants and a Phylogenetic tree was draw for it. In the next step, Converse points were recognized and by using Metal Detector Predicts v2.0 and DiANNA 1.1 web server, the sites containing metal binding and disulphide bonds were identified in amino acid sequence of mentioned protein. Also amino acids which contain Zinc-binding sit had been identified using PredZinc version 1.4, 2011-10-14. Results showed that the amino acid of this protein in plant Arabidopsis thaliana has the most similarity to the protein Nramp1 in plant Arabidopsis lyrata. 7 disulphide bonds and 34 Zinc-binding sites had also been detected in this protein.
