Title of article :
Chromatin Immunoprecipitation Reveals That the 180-bp Satellite Repeat Is the Key Functional DNA Element of Arabidopsis thaliana Centromeres
Author/Authors :
Jiang، Jiming نويسنده , , Nagaki، Kiyotaka نويسنده , , Dawe، R. Kelly نويسنده , , Talbert، Paul B. نويسنده , , Zhong، Cathy Xiaoyan نويسنده , , Henikoff، Steven نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2003
Abstract :
The centromeres of Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.
Keywords :
Ectomycorrhizae , Indicator species , Oligotrophic soils , N deposition , Pine barrens
