Author/Authors :
Nikravesh، Mohammad Reza نويسنده , , Jalali، Mehdi نويسنده , , Ghafaripoor، Hossein Ali نويسنده Associate Professor of Anatomy, School of Medicine , , Sanchooli، Javad نويسنده Associate Professor of Anatomy, School of Medicine , , Hamidi Alamdari، Darioush نويسنده , , Mohammadi ، Shabnam نويسنده Department of Anatomy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. , , Seghatoleslam، Masoomeh نويسنده PhD Student of Anatomy, Department of Anatomy and Cell Biology, School of Medicine ,
Abstract :
Introduction: Nowadays, the important role of stem cells in treatment of many diseases such as stoke is well
known. Stem cells derived from either bone marrow or cord bloods are good sources for tissues replacement after
post embryonic injuries.
Methods: Fourteen-day-old Wistar rats were used in this study. Rats were subjected to internal carotid artery
occlusion for 30 minutes. Then, animals were received intravenously 2×105 Bromo Deoxy Uridine (BRDU)
labeled- cord blood stem cells (CBSCs). Rats with hypoxic conditions that were not received any injection were
assumed as a sham group. Intact animals who did not receive any injection or surgeries were used as a control
group.
Results: Our results were evaluated according to behavioral tests and immunohistochemistry of the brain especially
frontal cortex of the control, sham and experimental groups. Behavioral recovery was observed in the experimental
group compared to the either the sham or the control group. In addition, histological studies demonstrated a
reduction in ischemic cells in the experimental group compared to the sham group.
Conclusion: Intravenous transplantation can be a future line in treatment of infants with hypoxic who are exposed to
irreversible damages.
