Monoclonal and polyclonal antibodies against fish vitellogenin for use in pollution monitoring

Two monoclonal antibodies (KB-1 and BN-5) and one polyclonal antibody (AA-1) were prepared against purified vitellogenin (Vtg) from Atlantic salmon (Salmo salar), and tested for their ability to bind Vtg from plasma of various other fish species. The MAb KB-1 was found to bind specifically to Vtg from the Salmo species, Atlantic salmon (Salmo salar) and brown trout (Salmo trutta). The MAb BN-5 showed a much wider cross-reactivity, binding to Vtg from both Salmoniformes species (Atlantic salmon, brown trout, rainbow trout and Arctic charr) and Pleuronectiformes species (turbot and halibut). The widest crossreactivity was observed with the polyclonal antibody AA-1 which, in addition to the species recognized by the MAb BN-5, also bound to Vtg from Atlantic cod (Gadiformes).