Author/Authors :
Ghobakhloo، M نويسنده Plant Protection Department, College of Agriculture & Natural Resources, University of Tehran, Karaj. , , Mosahebi، Gh.H نويسنده Plant Protection Department, College of Agriculture & Natural Resources, University of Tehran, Karaj. , , Kouhi Habibi، M نويسنده Plant Protection Department, College of Agriculture & Natural Resources, University of Tehran, Karaj , , Javan Nikkhah، M نويسنده Plant Protection Department, College of Agriculture & Natural Resources, University of Tehran, Karaj , , AHMADI، M.R. نويسنده ,
Abstract :
ABSTRACT: Sclerotiniasclerotiorum the causal agent of sclerotinia stem rot is one of the most important fungal plant pathogens with global distribution. Broad host range and causing severe damage on crops annually, has been increased the incentive for further study of this species. Nowadays possibility of using mycoviruses as modern strategies of fungal biological control is important. From agricultural perspectives, mycoviruses may contribute to sustainable agriculture as biological control agents.The presence of viral dsRNA were investigated in 50 isolates of S. sclerotiorum. Isolates obtained from different hosts including lettuce, sunflower, cabbage, rape-seed, soybean, tobacco and Artemisia in north of Iran. The dsRNA molecules were separated using STE buffer, Phenol, Chloroform and column chromatography of cellulose (CF-11). Out of 50, 23 isolates were detectedwhich have dsRNAmolecules.This is the first report of S. sclerotiorumdsRNAs from Iran. The impact ofmycoviruseson themorphologicalcharacteristics offungus was studied. ThedsRNA-containing had less mycelium linear growth thandsRNA-free isolates.
