Author/Authors :
Alizadeh، H. نويسنده His research field is antimicrobial agents and microbial resistance, working at the Young Researchers Club. , , Salouti، M. نويسنده Department of Biology, Faculty of Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran , , Shapouri، R. نويسنده head of the Microbiology Department and the Biology Research Center of the Islamic Azad University, ,
Abstract :
Brucellosis is a zoonosis disease of worldwide importance among animal and humans. The most
important virulence factor of Brucella spp. is related to its intramachrophage survival. On the other hand,
because of the side effects of the current brucellosis treatment regime, it is necessary to find out new
antimicrobial agents to treat the disease. The aim of this study is to investigate the antibacterial effect
of silver nanoparticles against Brucella under an intramacrophage condition. A well diffusion agar test
showed the activity of silver nanoparticles against Brucella melitensis 16M. Macrodilution experiments
demonstrated the MIC and MBC of silver nanoparticles 4 and 6 ppm, respectively. The incubation time
study showed that silver nanoparticles were able to kill bacteria within 40 min after the reaction.
The cell culture study revealed that silver nanoparticles have effective antimicrobial activity inside the
macrophage cells in concentrations of 46 ppm and the findings showed that one may consider silver
nanoparticles as a new nano drug against intramachrophage Brucella melitensis 16M.
