Author/Authors :
Ali ، S. M. نويسنده Foot and Mouth Disease Department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt. , , Ismail ، A. H. نويسنده Foot and Mouth Disease Department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt. , , Soliman ، E. M. نويسنده Central Laboratory for Evaluation of Vet. Biologics, Egypt. , , Mostafa، Hanaa A. نويسنده Central Laboratory for Evaluation of Vet. Biologics, Egypt. ,
Abstract :
Foot and mouth disease virus (FMDV) Type SAT-2/Egypt/2012 isolated from El-Gharbiya Governorate was propagated six serial passages into monolayer BHK21 cell line. Growth curve of the infected virus, development of complement fixing (CF) antigens and 146s content in infected BHK21 cells incubated at 2 different temperature (35?C and 37?C) were studied using the 6th virus passage. Maximum infectivity titer with 100% CPE of the infected cells was detected 21 hours post inoculation at both incubation temperatures. The highest complement fixing antigens titer and 146S protein content of the same strain was recorded 18 hours post infection at both different incubation temperatures. So the best time of choice for virus harvest is 18hrs. post inoculation at 37?C incubation.
