Author/Authors :
Nouzha ، H. نويسنده Laboratory of microbiology, Veterinary Department, Institute of Veterinary and Agricultural Sciences, Hadj Lakhdar University, 05000-Batna, Algeria. , , Ammar، Mohammad A. نويسنده , , Bakir، M. نويسنده Laboratory ESPA, Department of Veterinary Science, Institute of Veterinary Science and Agricultural Science, University Hadj Lakhdar, Algeria. , , Ahmed ، K. L. نويسنده Laboratory of microbiology, Department of Medical Science, Hadj Lakhdar University, 05000-Batna, Algeria. ,
Abstract :
Mycoplasma gallisepticum infection is commonly designated as chronic respiratory disease in chickens. It is the most pathogenic avian Mycoplasma; however, strains may differ markedly in virulence. In this study, the technical performance of culture, a commercially available polymerase chain reaction (PCR) test and rapid plate agglutination (SPA) test were compared for the detection of Mycoplasma gallisepticum infections from 18 birds. Results showed a high percentage of positive samples of both culture and PCR tests (72.22% and 63.63% respectively). SPA showed a less positive rate (61.11%). the utilization of SPA towards MG diagnosis is limited by its reduced specificity and the high incidence of false positives Contradictory to other studies, bacteriology was more sensitive than PCR. Several studies support strongly the use of the PCR as a technique for the diagnosis of Mycoplasma infections since this technique is more sensitive than serology and culture. This study showed that it is not advisable to rely completely on one test (system) only.
