Title of article :
Hexadecane-degradation by Teskumurella and Stenotrophomonas Strains Isolated From Hydrocarbon Contaminated Soils
Author/Authors :
Tebyanian، Hamid نويسنده Department of Microbiology, Science and Research Branch, Islamic Azad University, Kerman, IR Iran , , Hassanshahian، Mehdi نويسنده , , Kariminik، Ashraf نويسنده Department of Microbiology, Islamic Azad University, Kerman, IR Iran ,
Issue Information :
فصلنامه با شماره پیاپی 25 سال 2013
Pages :
7
From page :
1
To page :
7
Abstract :
Background: Petroleum hydrocarbons are mix compounds and divided into four groups: Saturates, Aromatics, Resins and Asphalten. Among various phases of crude-oil, the alkanes with medium length chain are favorable substrates that rapidly degraded, although short-chain alkanes are very toxic and long-chain alkanes have low solubility in water that reduce its bioavailability and make resistant to biodegradation. Objectives: The main goal of this study is the isolation, molecular identification and degradation properties of hexadecane degrading bacteria from contaminated soils. Materials and Methods: In this research to isolate aliphatic degrading bacteria, sampling from hydrocarbon contaminated soil with of petroleum reservoirs regions, Tehran were performed. Alkane degrading bacteria were isolated by enrichment in Bushnel-Hass medium with hexadecane as sole source of carbon and energy. The isolated strains were identified by amplification of 16S rDNA gene and sequencing. Alkane hydroxylase gene (alk-B) was identified in all strains by PCR with specific primers. Results: Among 8 strains three strains with high growth rate on hexadecane selected for further study. These three selected strains identified as Stenotrophomonas maltophilia strain M2, S. maltophilia strain Q2 and Tsukamurella tyrosinosolvens strain Q3. In comparison to the other bacteria, these bacterial strains can degrade hexadecane 2 times more; with a high emulsification activity. Conclusions: The 2.5% concentration of hexadecane was the best concentration that supports the growth of these strains. Among these strain T. tyrosinosolvens strain Q3 was the best strain for biodegradation of hexadecane. Alk-B gene was identified in all strains.
Journal title :
Jundishapur Journal of Microbiology (JJM)
Serial Year :
2013
Journal title :
Jundishapur Journal of Microbiology (JJM)
Record number :
945114
Link To Document :
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