Author/Authors :
Atsamo ، A. D. نويسنده Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Science, University of Dschang , Cameroon. , , Nene-Bi ، S. A. نويسنده Laboratoy of Animal Physiology, UFR of Biosciences, Felix Houphouet Boigny University , P.O. Box 582, Abidjan 22, Ivory Coast. , , Kouakou ، K. L. نويسنده Laboratory of Animal Physiology,pharmacology and pharmacopoeia,UFR of Natural Sciences,University of Abobo-Adjame,P.O. Box 801, Abidjan 02,Ivory Coast , , Fofie ، K. C. نويسنده Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Science, University of Dschang , Cameroon. , , Nyadjeu ، P. نويسنده Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Science, University of Dschang , Cameroon. , , Watcho ، P. نويسنده Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Science, University of Dschang , Cameroon. , , Datte ، J. Y. نويسنده Laboratory of Pharmacology and Nutrition, UFR of Biosciences, Felix Houphouet Boigny University, P.O. Box 582, Abidjan 22, Ivory Coast. , , Kamanyi ، A. نويسنده Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Science, University of Dschang , Cameroon. , , Nguelefack ، T. B. نويسنده Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Science, University of Dschang , Cameroon. ,
Abstract :
The present study was undertaken to investigate the cardiovascular and the in vitro antioxidant activities of the methanol extract of the stem bark of Erythrina senegalensis (EMES), a plant used traditionally in Cameroon against arterial hypertension. The cardiovascular effects of EMES (11-44 mg/kg) were evaluated in vivo on normotensive rat arterial blood pressure and on rabbit’s electrocardiogram (5.5- 55 mg/kg) while the in vitro study (10-10 - 1 mg/ml) on myocardial contractile performance was evaluated on rat isolated heart. The antioxidant activities of EMES (1-1000 µg/ml) were investigated on 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2)-induced haemolysis, nitric oxide (NO) production, as well as its reducing power. The intravenous injection of EMES provoked in anaesthetized normotensive rats, a dose dependent immediate reduction in arterial hypertension. The mean arterial blood pressure dropped by 11.29, 19.84 and 24.42 % at respective doses of 11, 22 and 44 mg/kg. When administered intravenously in rabbit EMES significantly reduced the heart rate, amplitudes of P wave and QRS complex while significantly increasing the duration of the QT interval. EMES exhibited a concentration dependent reducing power, antiradical activity on DPPH (EC50 of 46.9 ?g/ml) and a significant (p < 0.001) antioxidant effect on H2O2-induced haemolysis. EMES also substantially increase NO production. In conclusion, EMES exert immediate hypotensive effect which might be attributed to its negative inotropic and chronotropic effects. Its in vitro antioxidant activities could be beneficial for the treatment of cardiovascular ailments.
