Decrease Effect of Vitis Vinifera Leave Hydro-Alcoholic Extract on Reproductive Parameters In Adult Normal Male Rats

Grape leaves are used in a traditional food and for treatment of diarrhea, bleeding; and also reported that administered grape was good to treatment of constipation, gout and etc. So, the aim of this study was to investigate the decrease effect of grape leave hydro-alcoholic extract (GLHE) on reproductive parameters in normal male rats. Its leaves were dried and powdered, and mixed with ethanol, after prepared extract, 250 and 500 mg/kg/B.W of this extract was administered into two experimental groups, control received 1ml Nacl 0.9% by gavage for 42 days. 24h after last dosage, all rats anesthetized, then, their couda epididymis and testis dissected on and kept its in Nacl 0.9% separately, for measured sperm motility, total sperm and daily sperm production, then counted them by microscope. The results showed a significant decrease in epididymal, testicular sperm counts and daily production, likewise, percent of sperm-progressive and non-progressive motility vs. control. So, GLHE was significantly decreased effect on final body and ventral prostate weights vs. control, also weight of cauda epididymis in test group I was significantly decrease vs. control. So, it seems that administration of this extract affects on some of fertility parameters and decreases spermatogenesis in the rats.