Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species

Pandurangan، Ashok Kumar نويسنده Department of Biochemistry, University of Madras, Chennai, Tamil Nadu, India , , Ganapasam، Sudhandiran نويسنده Department of Biochemistry, University of Madras, Chennai, Tamil Nadu, India ,

فصلنامه با شماره پیاپی 16 سال 2013

6

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Background: Colorectal cancer, a major health concern worldwide, is the third most common form of cancer and second leading cause of cancer-related deaths. The flavonoids are naturally occurring diphenylpropanoids ubiquitous in plant foods and important components of the human diet. Luteolin, a bioflavonoid, possesses many beneficial effects including antioxidant, anti-inflammatory, anti-allergic activities. Methods:We used the HCT-15 colon adenocarcinoma cell line in this study. Cells were treated with luteolin (100 µM). Results: Membrane damage markers such as alkaline phosphatase and lactate dehydrogenase were analyzed in a time-dependent manner. Luteolin increased reactive oxygen species in a time-dependent manner. DNA damage, a hallmark of apoptosis, was induced by luteolin as analyzed by agarose gel electrophoresis. Conclusion: Luteolin acts as a potential cytotoxic agent that can be used to treat colorectal cancer.

Middle East Journal of Cancer (MEJC)

2013

Middle East Journal of Cancer (MEJC)

945981