Studies of the action pattern on potato starch of the decycling maltodextrinase from Flavobacterium sp. no. 92

The action pattern on potato starch of the decycling maltodextrinase from Flavobacterium sp. no. 92 was studied, and its limit dextrins were analysed. The enzymic activity of the maltodextrinase with starch and its components (1% solutions) proved to be in the order amylose > whole starch ⪢ amylopectin. Revealed by the Michaelis constant of 9 g.L−1, the enzyme displayed a fairly low affinity for starch, but the maximum hydrolysis rate was found to be around 165 U/mg of protein. The initial products of starch degradation comprised, besides some maltotriose to maltopentaose, mainly maltose, which originated predominantly from am Intermediate larger fragments with a of 48, amounting to roughly 27% of amylopectin, and comprising highly branched clusters, were analysed b