Analysis of the polysaccharide components of the lipopolysaccharide fraction of Pseudomonas caryophylli

The composition of the polysaccharide moieties of the lipopolysaccharide (LPS) fraction of Pseudomonas caryophylli is described. Two homopolysaccharide chains, the major one built up of (1 → 7)-linked α-caryophyllose [3,6,10-trideoxy-4-C-(d-glycero-1-hydroxyethyl)-d-erythro-d-gulo-decose] residues and the minor one made up of (1 → 7)-linked β-caryose (4,8-cyclo-3,9-dideoxy-l-erythro-d-ido-nonose) residues, were identified in the LPS fraction. In addition, experimental evidence of a third polysaccharide fraction mainly composed of heptose and glucose is also reported. The structural analysis was performed by chemical and spectroscopic methods.