1H and 13C NMR analysis of the pentasaccharide Galβ(1 → 4)GlcNAcβ(1 → 3)-[GlcNAcβ(1 → 6)]Galβ(1 → 4)GlcNAc synthesized by the mid-chain β-(1 → 6)-d-N-acetylglucosaminyltransferase of rat serum

Chemical shifts and coupling constants of completely assigned 1H and 13C NMR spectra at 500 MHz, as well as ROESY and HMBC connectivities were used to establish the structure of the pentasaccharide Galβ(1 → 4)GlcNAcβ(1 → 3)[GlcNAcβ(1 → 6)]Galβ(1 → 4)GlcNAc, synthesized by the action of the mid-chain β-(1 → 6)-d-N-acetylglucosaminyltransferase of rat serum from UDP-GlcNAc and the linear tetrasaccharide Galβ(1 → 4)GlcNAcβ(1 → 3)Galβ(1 → 4)GlcNAc. © 1997 Elsevier Science Ltd.