Structure of the O-specific polysaccharide of Salmonella enterica ssp. arizonae O50 (Arizona 9a,9b)

On the basis of sugar and methylation analysis, selective removal of 3,6-dideoxy-l-xylo-hexose (colitose, Col), 1H and 13C NMR spectroscopy, including 1D NOE, 2D COSY, and 2D H-detected 1H, 13C heteronuclear multiple-quantum coherence (HMQC), the following structure of the repeating unit of the O-specific polysaccharide of Salmonella enterica ssp. arizonae O50 (Arizona 9a,9b) was established: The O-antigen studied includes a trisaccharide fragment α-Col p-(1 → 2)-β-d-Gal p-(1 → 3)-β-d-Glc pNAc, which is a colitose (‘3-deoxy-l-fucose’) analogue of the Lewisd (precursor) blood group antigen.