Primary structure of seven sulfated oligosaccharide-alditols released by reductive β-elimination from oviducal mucins of Rana temporaria characterization of the sequence HSO3(3)GlcA(β1–3)Gal

The mucins isolated from Rana temporaria egg jelly coats were found to be composed of Gal, Fuc, GlcNAc, Ga1NAc and GlcA acid. The primary structure of seven sulfated oligosaccharide-alditols was obtained by 1D/2D NMR analyses (1H-13C). The results show the presence of the sulfated monosaccharides HSO3(3)Gal, HSO3(6)GlcNAc and HSO3(3)GlcA. The sequence HSO3(3)GlcA(β1–3)Gal, which constitutes the major determinant of the HNK-1 oncofoetal epitope, was characterized.