Identification of an α-d-Manp-(1 → 8)-Kdo disaccharide in the inner core region and the structure of the complete core region of the Legionella pneumophila serogroup 1 lipopolysaccharide

A disaccharide α-d-mannopyranosyl-(1 → 8)-3-deoxy-d-manno-octulosonic acid [α-d-Manp-(1 → 8)-Kdo] was released by mild acid degradation of Legionella pneumophila serogroup 1 (strain Philadelphia 1) lipopolysaccharide (LPS) and identified using NMR spectroscopy and GLC-MS of derived products. These data, together with methylation analysis of the native LPS and previously reported data [Y.A. Knirel, H. Moll, and U. Zähringer, Carbohydr. Res., 293 (1996) 223–234], allowed elucidation of the complete core region of the LPS as having the following nonasaccharide structure: A disaccharide α-d-mannopyranosyl-(1 → 8)-3-deoxy-d-manno-octulosonic acid [ α-d-Man p-(1 → 8)-Kdo] was released by mild acid degradation of Legionella pneumophila serogroup 1 (strain Philadelpha 1) lipopolysaccharide (LPS) and identified using NMR spectroscopy and GLC-MS of derived products.