Structural studies of an exopolysaccharide produced by Alteromonas macleodii subsp. fijiensis originating from a deep-sea hydrothermal vent

The structure of the exopolysaccharide produced by Alteromonas macleodii subsp. fijiensis recovered from a deep-sea hydrothermal vent has been investigated. By means of chemical analysis and NMR studies, the repeating unit of the polymer was deduced to be a branched hexasaccharide with the structure shown. Article Outline 1. Introduction 2. Results and discussion 2.1. Isolation, purification and composition of the polysaccharide 2.2. Glycosyl-linkage analysis 2.3. Position of the uronic acid residues 2.4. NMR studies 3. Experimental 3.1. Bacterial strain 3.2. Production and isolation of the polysaccharide 3.3. Purification of the polysaccharide 3.4. General methods 3.5. Constituent analysis 3.6. Methylation analysis 3.7. β-Elimination 3.8. NMR spectroscopy Acknowledgements References