Structural heterogeneity of the sialic-acid-containing oligosaccharides from the lipopolysaccharide of Hafnia alvei strain 2 as detected by FABMS studies

The structure of four oligosaccharide fractions from the Hafnia alvei strain 2 lipopolysaccharide (LPS) have been assigned by FABMS. This approach corroborates data previously established by NMR spectroscopy for the major oligosaccharides in these fractions [A. Gamian, E. Romanowska, U. Dabrowski, J. Dabrowski, Biochemistry 30 (1991) 5032–5038; E. Katzenellenbogen, A. Gamian, E. Romanowska, U. Dabrowski, J. Dabrowski, Biochem. Biophys. Res. Commun. 194 (1993) 1058–1064; N. Ravenscroft, A. Gamian, E. Romanowska, Eur. J. Biochem. 227 (1995) 889–896]. In addition, the MS/MS with B/E linked scan technique allowed the detection of an additional oligosaccharide with the structure: