High-performance anion-exchange chromatography of sugar and glycerol phosphates on quaternary ammonium resins

Twenty-six sugar and glycerol phosphates were separated by high-performance anion-exchange chromatography on an alkyl quaternary ammonium column at pH 6.0 with 0.1 M sodium acetate eluant. Capacity, asymmetry, and response factors of the compounds vary widely. Secondary acidic dissociation constants of 14 of these phosphates were determined in an attempt to explain the separation mechanism.