Author/Authors :
Martin Albert، نويسنده , , Werner Repetschnigg، نويسنده , , J?rg Ortner، نويسنده , , Joseph Gomes، نويسنده , , Bernhard J. Paul، نويسنده , , Carina Illaszewicz، نويسنده , , Hansj?rg Weber، نويسنده , , Walter Steiner، نويسنده , , Karl Dax، نويسنده ,
Abstract :
A fast method for the simultaneous detection of different glycosidolytic activities in commercially available enzyme preparations and crude culture filtrates was found in using, as substrate, a mixture of different glycosyl fluorides and 19F NMR spectroscopy as a screening technique. Accompanying studies regarding the hydrolytic stability of these fluorides in various buffer systems, as well as conditions of their long-term storage, were carried out. A simple procedure for the preparation of β-d-mannopyranosyl fluoride in gram quantities is given.
Keywords :
Glycosyl fluorides , Glycosidases , 19F NMR spectroscopy , ?-d-Mannopyranosyl fluoride
