Enzymatic synthesis of fucose-containing disaccharides employing the partially purified α-l-fucosidase from Penicillium multicolor Original Research Article

The α-l-Fucp-(1→3)-d-GlcpNAc disaccharide structure is a vital core unit of the oligosaccharide components of glycoconjugates isolated from human milk and blood group substances. α-l-Fucosidase from Penicillium multicolor catalyses the transfer of l-fucose from donor structures such as α-l-FucpOpNP and α-l-FucpF to various GlcpNAc derivatives and Glcp, forming α-(1→3) linkages. The synthesis of several biologically relevant disaccharides including α-l-Fucp-(1→3)-α-d-GlcpNAcOMe, α-l-Fucp-(1→3)-α-d-GlcpNAcOAll, α-l-Fucp-(1→3)-β-d-GlcpNAcOAll, α-l-Fucp-(1→3)-d-GlcpNAc and α-l-Fucp-(1→3)-d-Glcp has been achieved in up to 34% yields by application of this enzyme.