• Title of article

    A very efficient method to cleave Lipid A and saccharide components in bacterial lipopolysaccharides

  • Author/Authors

    E Manzo، نويسنده , , A Molinaro، نويسنده , , E Bedini، نويسنده , , C De Castro، نويسنده , , M Parrilli، نويسنده ,

  • Issue Information
    هفته نامه با شماره پیاپی سال 2001
  • Pages
    4
  • From page
    339
  • To page
    342
  • Abstract
    A novel mild procedure for the selective cleavage of ketosidic linkages is developed using ceric ammonium nitrate (CAN) in anhydrous N,N-dimethylformamide. Its application to lipopolysaccharides (LPS) is very significant because in the so far investigated LPS, the connection between the Lipid A region and the oligo(poly)saccharide part is always a keto-sugar. This procedure has been tested on LPS of Escherichia coli which contains Kdo as a linker between Lipid A and OPS and on Acinetobacter haemoliticus which contains d-glycero-d-talo-2-octulopyranosonic acid (Ko) as a linker and it performed efficiently in both cases.
  • Keywords
    Ketosidic cleavage , d-glycero-d-talo-2-Octulopyranosonic acid , Kdo , Gram-negative bacteria , O-antigen , Endotoxin , Ceric ammonium nitrate
  • Journal title
    Carbohydrate Research
  • Serial Year
    2001
  • Journal title
    Carbohydrate Research
  • Record number

    963263