NMR-based identification of cell wall anionic polymers of Spirilliplanes yamanashiensis VKM Ac-1993T

The cell wall of Spirilliplanes yamanashiensis VKM Ac-1993T contains four anionic polymers, viz., three teichoic acids and a sugar-1-phosphate polymer. The following are the structures of the teichoic acids: poly[-6-β-d-glucopyranosyl-(1→2)-glycerol phosphate] (PI), 1,3-poly(glycerol phosphate) bearing N-acetyl-α-d-glucosamine residues at O-2 (70%) (PII), and poly[-6-N-acetyl-α-d-glucosaminyl-(1→2)-glycerol phosphate] (PIII). The repeating unit of the fourth polymer (PIV) has the structure of -6-α-d-GlcpNAc-(1→6)-α-d-GlcpNAc-1-P- with a 3-O-methyl-α-d-mannopyranosyl residues at position 3 of some 6-phosphorylated N-acetylglucosamine residues (50%). Polymers PI, PIII and PIV have not hitherto been found in prokaryotic cell walls.