The structure of the glycopeptides from the fish pathogen Flavobacterium columnare Original Research Article

Proteolytic digestion of the phenol–water extraction product of the fish pathogen Flavobacterium columnare afforded a mixture of glycopeptides in which the oligosaccharide moiety was an unusual hexasaccharide composed of 4-O-methyl-2-acetamido-2-deoxy-d-glucuronic acid (GlcNAcA), d-glucuronic acid (d-GlcA), 2,3-di-O-acetyl-d-xylose (d-Xyl), 2-O-methyl-d-glucuronic acid (d-GlcA), d-mannose (d-Man), and 2-O-methyl-l-rhamnose (l-Rha). By the application of high-resolution 1D and 2D NM