Enzymatic supported synthesis of lacto-N-neotetraose using dendrimeric polyethylene glycol Original Research Article

The lacto-N-neotetraose tetrasaccharide was synthesized on a new dendrimeric support, based on polyethylene glycol. Starting from 1-thio-β-d-lactose, the trisaccharide (2-acetamido-2-deoxy-β-d-glucopyranosyl)-(1 → 3)-O-β-d-galactopyranosyl-(1 → 4)-1-thio-β-d-glucopyranose was obtained using Neisseria meningitidis β-(1 → 3)-N-acetylglucosaminyltransferase according to a soluble synthesis approach, bound on the support and galactosylated using the milk β-(1 → 4)-galactosyl transferase to give after cleavage the tetrasaccharide lacto-N-neotetraose.