Author/Authors :
Sofʹya N. Senchenkova، نويسنده , , Andrei V. Perepelov، نويسنده , , Maciej Cedzynski، نويسنده , , Anna S. Swierzko، نويسنده , , Andrzej Ziolkowski، نويسنده , , Alexander S. Shashkov، نويسنده , , Wieslaw Kaca، نويسنده , , Yuriy A. Knirel، نويسنده , , Per-Erik Jansson، نويسنده ,
Abstract :
A highly phosphorylated O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Proteus mirabilis O41 followed by GPC. The initial and dephosphorylated polysaccharides and phosphorylated products from two sequential Smith degradations were studied by 1H, 13C and 31P NMR spectroscopy and ESI-MS. The O-polysaccharide was found to have a tetrasaccharide repeating unit containing one ribitol phosphate (presumably d-Rib-ol-5-P) and two ethanolamine phosphate (Etn-P) groups, one of which is present in the stoichiometric amount and the other in a nonstoichiometric amount. The following structure of the O-polysaccharide was established:
Keywords :
Ethanolamine phosphate , Ribitol phosphate , O-polysaccharide structure , Proteus mirabilis , Lipopolysaccharide
