Author/Authors :
Kenji Osumi، نويسنده , , Yoshitaka Makino، نويسنده , , Eri Akaike، نويسنده , , Takashi Yamanoi، نويسنده , , Mamoru Mizuno، نويسنده , , Midori Noguchi، نويسنده , , Toshiyuki Inazu، نويسنده , , Kenji Yamamoto، نويسنده , , Kiyotaka Fujita، نويسنده ,
Abstract :
We found that the recombinant endo-β-N-acetylglucosaminidase of Mucor hiemalis (Endo-M) expressed in Candida boidinii had the transglycosylation activity of transferring a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide to the acceptor (p-nitrophenyl 2-acetamido-2-deoxy-β-d-glucopyranoside) in a good yield of 43%.
Keywords :
Endo-?-N-acetylglucosaminidase , Bisecting hybrid-type oligosaccharide , Transglycosylation , Endo-M
