Synthesis and antibacterial activity of mechanism-based inhibitors of KDO8P synthase and DAH7P synthase Original Research Article

KDO8PS (3-deoxy-d-manno-2-octulosonate-8-phosphate synthase) and DAH7PS (3-deoxy-d-arabino-2-heptulosonate-7-phosphate synthase) are attractive targets for the development of new anti-infectious agents. Both enzymes appear to proceed via a common mechanism involving the reaction of phosphoenolpyruvate (PEP) with arabinose 5-phosphate or erythrose-4-phosphate, to produce the corresponding ulosonic acids, KDO8P and DAH7P, respectively. The synthesis of new inhibitors closely related to the supposed tetrahedral intermediate substrates for the enzymes is described. The examination of the antibacterial activity of these derivatives is reported.