Enzymatic synthesis of oligosaccharides from branched cyclodextrins

6″-α-Maltosyl-maltotriose and 6″-α-d-glucosyl-maltotriose were prepared from Novamyl™ degradation of 6-O-α-maltosyl-α-cyclodextrin and 6-O-α-d-glucosyl-α-cyclodextrin, respectively. NMR spectroscopy was used to elucidate their structural identities, in a combination of COSY experiments. Further, a mechanism for the degradation was proposed based on the Novamyl active site geometry.