Physicochemical properties and antitumor activities of water-soluble native and sulfated hyperbranched mushroom polysaccharides Original Research Article

A water-soluble hyperbranched β-glucan, coded as TM3b, extracted from sclerotia of an edible fungus (Pleurotus tuber-regium) was fractioned into eight fractions coded as F1–F8 by a nonsolvent addition method. Five fractions were treated with chlorosulfonic acid at 35 °C to synthesize successfully sulfated derivatives coded as S-F2, S-F3, S-F4, S-F5, and S-F8 with degree of substitution of 0.28–0.54. The 13C NMR results of these sulfated β-glucans indicated that while the C-6 position was fully substituted, C-2, C-3, and C-4 were only partially substituted by the sulfate groups. The weight-average molecular weights (Mw) and intrinsic viscosities ([η]) of the native and sulfated TM3b fractions were determined using multi-angle laser light scattering and viscometry in 0.15 M aq NaCl at 25 °C,