Author/Authors :
Sophie Blanchard، نويسنده , , Sylvie Armand، نويسنده , , Pedro Couthino، نويسنده , , Shamkant Patkar، نويسنده , , Jesper Vind، نويسنده , , Eric Samain، نويسنده , , Hugues Driguez، نويسنده , , Sylvain Cottaz، نويسنده ,
Abstract :
Four Humicola insolens Cel7B glycoside hydrolase mutants have been evaluated for the coupling of lactosyl fluoride on O-allyl NI-acetyl-2II-azido-β-chitobioside. Double mutants Cel7B E197A H209A and Cel7B E197A H209G preferentially catalyze the formation of a β-(1→4) linkage between the two disaccharides, while single mutant Cel7B E197A and triple mutant Cel7B E197A H209A A211T produce predominantly the β-(1→3)-linked tetrasaccharide. This result constitutes the first report of the modulation of the regioselectivity through site-directed mutagenesis for an endoglycosynthase.
Keywords :
Cellulase , Regioselectivity , Glycosynthase , Enzymatic synthesis
